PKN1 antibody (Internal Region)

Catalog No. ABIN7223532

The Rabbit Polyclonal anti-PKN1 antibody has been validated for WB and ELISA. It is suitable to detect PKN1 in samples from Human, Mouse and Rat.

Quick Overview for PKN1 antibody (Internal Region) (ABIN7223532)

Target: PKN1 (Protein Kinase N1 (PKN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PKN1 Antibody

Binding Specificity: Internal Region

Purpose: PKN Polyclonal Antibody

Specificity: PKN Polyclonal Antibody detects endogenous levels of PKN protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PKN

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Alternative Name: PKN

Background: Rabbit Anti-PKN Polyclonal Antibody,PKN1, PAK1, PKN, PRK1, PRKCL1, Serine/threonine-protein kinase N1, Protease-activated kinase 1, PAK-1, Protein kinase C-like 1, Protein kinase C-like PKN, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein,The protein encoded by PKN1 (protein kinase N1) belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.,serine threonine-protein kinase N1

Molecular Weight: observerd band 105kDa

Gene ID: 5585

UniProt: Q16512