POFUT1 antibody (AA 300-380)

Catalog No. ABIN7220730

This anti-POFUT1 antibody is a Rabbit Polyclonal antibody detecting POFUT1 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for POFUT1 antibody (AA 300-380) (ABIN7220730)

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POFUT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POFUT1 Antibody

Binding Specificity: AA 300-380

Purpose: O-FucT-1 Polyclonal Antibody

Specificity: O-FucT-1 Polyclonal Antibody detects endogenous levels of O-FucT-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human O-FucT-1 at AA range: 300-380

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for POFUT1

Target: POFUT1 (Protein O-Fucosyltransferase 1 (POFUT1))

Alternative Name: O-FucT-1

Background: Rabbit Anti-O-FucT-1 Polyclonal Antibody,POFUT1, FUT12, KIAA0180, GDP-fucose protein O-fucosyltransferase 1, Peptide-O-fucosyltransferase 1, O-FucT-1,POFUT1 encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms.,GDP-fucose protein O-fucosyltransferase 1

Molecular Weight: observerd band 44kDa

Gene ID: 23509

UniProt: Q9H488

Pathways: Notch Signaling, SARS-CoV-2 Protein Interactome