PPP1CA antibody

Catalog No. ABIN7223348

The Rabbit Polyclonal anti-PPP1CA antibody (ABIN7223348) specifically detects PPP1CA in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PPP1CA antibody (ABIN7223348)

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1CA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PPP1CA Antibody

Purpose: Rabbit Anti-PP1α Polyclonal Antibody

Specificity: PP1α Polyclonal Antibody detects endogenous levels of PP1α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PP1alpha around the non-phosphorylation site of T320

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPP1CA

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Alternative Name: PP1alpha

Background: PPP1CA, PPP1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1AThe protein encoded by PPP1CA (protein phosphatase 1 catalytic subunit alpha) is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for PPP1CA.

Molecular Weight: 37kD

Gene ID: 5499

UniProt: P62136

Pathways: M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism