PPP1R15B antibody (Internal Region)

Catalog No. ABIN7218646

This Rabbit Polyclonal antibody specifically detects PPP1R15B in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PPP1R15B antibody (Internal Region) (ABIN7218646)

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R15B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPP1R15B Antibody

Binding Specificity: Internal Region

Purpose: PPP1R15B Polyclonal Antibody

Specificity: PPP1R15B Polyclonal Antibody detects endogenous levels of PPP1R15B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PPP1R15B

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PPP1R15B

Target: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Alternative Name: PPP1R15B

Background: Rabbit Anti-PPP1R15B Polyclonal Antibody,PPP1R15B, Protein phosphatase 1 regulatory subunit 15B,PPP1R15B encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism.,Protein phosphatase 1 regulatory subunit 15B

Molecular Weight: observerd band 78kDa

Gene ID: 84919

UniProt: Q5SWA1

Pathways: ER-Nucleus Signaling