PDP antibody (AA 180-260)

Catalog No. ABIN7230574

This anti-PDP antibody is a Rabbit Polyclonal antibody detecting PDP in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for PDP antibody (AA 180-260) (ABIN7230574)

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDP Antibody

Binding Specificity: AA 180-260

Purpose: PDP1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PDP1 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human PDP1 protein at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PDP

Target: PDP (Pyruvate Dehydrogenase Phosphatase (PDP))

Alternative Name: PDP1

Background: Rabbit Anti-PDP1 Polyclonal Antibody,[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, PDP 1, Protein phosphatase 2C, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1,Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported, one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by PDP1, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in PDP1 causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.,PDP1

Molecular Weight: observerd band 59kDa

Gene ID: 54704

UniProt: Q9P0J1