RAD9A antibody

Catalog No. ABIN7223890

The Rabbit Polyclonal anti-RAD9A antibody (ABIN7223890) specifically detects RAD9A in ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAD9A antibody (ABIN7223890)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD9A Antibody

Purpose: Rabbit Anti-Rad9 Polyclonal Antibody

Specificity: Rad9 Polyclonal Antibody detects endogenous levels of Rad9 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rad9 around the non-phosphorylation site of S272

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: Rad9

Background: RAD9A, Cell cycle checkpoint control protein RAD9A, hRAD9, DNA repair exonuclease rad9 homolog ARAD9A (RAD9 checkpoint clamp component A) product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for RAD9A.

Gene ID: 5883

UniProt: Q96C41

Pathways: Positive Regulation of Response to DNA Damage Stimulus