RAP1GAP antibody (AA 460-540)

Catalog No. ABIN7216788

This Rabbit Polyclonal antibody specifically detects RAP1GAP in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for RAP1GAP antibody (AA 460-540) (ABIN7216788)

Target: RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAP1GAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RAP1GAP Antibody

Binding Specificity: AA 460-540

Purpose: Rabbit Anti-Rap1GAP Polyclonal Antibody

Specificity: Rap1GAP Polyclonal Antibody detects endogenous levels of Rap1GAP protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Rap1GAP at AA range: 460-540

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAP1GAP

Target: RAP1GAP (RAP1 GTPase Activating Protein (RAP1GAP))

Alternative Name: Rap1GAP

Background: RAP1GAP, KIAA0474, RAP1GA1, Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1RAP1GAP encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Molecular Weight: 73kD

Gene ID: 5909

UniProt: P47736