RASA1 antibody

Catalog No. ABIN7230946

This Rabbit Polyclonal antibody specifically detects RASA1 in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for RASA1 antibody (ABIN7230946)

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RASA1 Antibody

Purpose: Rabbit Anti-RASA1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RASA1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RASA1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RASA1

Target: RASA1 (RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1))

Alternative Name: RASA1

Background: Ras GTPase-activating protein 1, GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAPRAS P21 Protein Activator 1 encoded by RASA1 is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Molecular Weight: 115kD

Gene ID: 5921

UniProt: P20936

Pathways: Regulation of Actin Filament Polymerization, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals