RGS14 antibody (AA 100-180)

Catalog No. ABIN7219096

The Rabbit Polyclonal anti-RGS14 antibody (ABIN7219096) specifically detects RGS14 in WB, ELISA and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for RGS14 antibody (AA 100-180) (ABIN7219096)

Target: RGS14 (Regulator of G-Protein Signaling 14 (RGS14))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RGS14 Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-RGS14 Polyclonal Antibody

Specificity: RGS14 Polyclonal Antibody detects endogenous levels of RGS14 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RGS14 at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RGS14

Target: RGS14 (Regulator of G-Protein Signaling 14 (RGS14))

Alternative Name: RGS14

Background: RGS14, Regulator of G-protein signaling 14, RGS14RGS14 encodes a member of the regulator of G-protein signaling family. Regulator of G-protein signaling 14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Molecular Weight: 65kD

Gene ID: 10636

UniProt: O43566

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Platelet-derived growth Factor Receptor Signaling