RGS5 antibody (AA 60-140)

Catalog No. ABIN7225470

The Rabbit Polyclonal anti-RGS5 antibody is suitable to detect RGS5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for RGS5 antibody (AA 60-140) (ABIN7225470)

Target: RGS5 (Regulator of G-Protein Signaling 5 (RGS5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RGS5 Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-RGS5 Polyclonal Antibody

Specificity: RGS5 Polyclonal Antibody detects endogenous levels of RGS5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RGS5 at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RGS5

Target: RGS5 (Regulator of G-Protein Signaling 5 (RGS5))

Alternative Name: RGS5

Background: RGS5, Regulator of G-protein signaling 5, RGS5RGS5 (regulator of G-protein signaling 5) encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. RGS5 is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. RGS5 is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified.

Molecular Weight: 20kD

Gene ID: 8490

UniProt: O15539

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling