ARHGEF7 antibody (AA 280-360)

Catalog No. ABIN7225698

This anti-ARHGEF7 antibody is a Rabbit Polyclonal antibody detecting ARHGEF7 in WB, ELISA and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for ARHGEF7 antibody (AA 280-360) (ABIN7225698)

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARHGEF7 Antibody

Binding Specificity: AA 280-360

Purpose: PIXβ Polyclonal Antibody

Specificity: PIXβ Polyclonal Antibody detects endogenous levels of PIXβ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PIXbeta at AA range: 280-360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ARHGEF7

Target: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Alternative Name: PIXbeta

Background: Rabbit Anti-PIXβ Polyclonal Antibody,ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB, Nbla10314, Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85,ARHGEF7 (Rho guanine nucleotide exchange factor 7) encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for ARHGEF7.,Rho guanine nucleotide exchange factor 7

Molecular Weight: observerd band 80kDa

Gene ID: 8874

UniProt: Q14155

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation