RhoBTB1/2 (AA 30-110) antibody

Catalog No. ABIN7216832

This Rabbit Polyclonal antibody specifically detects RhoBTB1/2 in ELISA, IF, IHC and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for RhoBTB1/2 (AA 30-110) antibody (ABIN7216832)

Target: RhoBTB1/2

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-RhoBTB1/2 Polyclonal Antibody

Specificity: RhoBTB1/2 Polyclonal Antibody detects endogenous levels of RhoBTB1/2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human RhoBTB1/2 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: RhoBTB1/2

Background: RHOBTB1, KIAA0740, Rho-related BTB domain-containing protein 1, RHOBTB2, DBC2, KIAA0717, Rho-related BTB domain-containing protein 2, Deleted in breast cancer 2 gene protein, p83Rho related BTB domain containing 2 encoded by RHOBTB2 is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 83kD

Gene ID: 23221, 9886

UniProt: O94844, Q9BYZ6