RhoGEF p115 (AA 140-220) antibody

Catalog No. ABIN7216838

The Rabbit Polyclonal anti-RhoGEF p115 antibody is suitable to detect RhoGEF p115 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for RhoGEF p115 (AA 140-220) antibody (ABIN7216838)

Target: RhoGEF p115

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 140-220

Purpose: Rabbit Anti-RhoGEF p115 Polyclonal Antibody

Specificity: RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RhoGEF p115 at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: RhoGEF p115

Background: ARHGEF1, Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, p115-RhoGEF, p115RhoGEF, Sub1.5Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The rho guanine nucleotide exchange factor 1 may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Molecular Weight: 105kD

Gene ID: 9138

UniProt: Q92888