RPL7 antibody (AA 180-260)

Catalog No. ABIN7216866

The Rabbit Polyclonal anti-RPL7 antibody (ABIN7216866) specifically detects RPL7 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat and Cat samples.

Quick Overview for RPL7 antibody (AA 180-260) (ABIN7216866)

Target: RPL7 (Ribosomal Protein L7 (RPL7))

Reactivity: Human, Mouse, Rat, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RPL7 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-Ribosomal Protein L7 Polyclonal Antibody

Specificity: Ribosomal Protein L7 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L7 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Ribosomal Protein L7 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RPL7

Target: RPL7 (Ribosomal Protein L7 (RPL7))

Alternative Name: Ribosomal Protein L7

Background: RPL7, 60S ribosomal protein L7Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL7 encodes a ribosomal protein that is a component of the 60S subunit. 60S ribosomal protein L7 belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Molecular Weight: 32kD

Gene ID: 6129

UniProt: P18124