RPS3 antibody (AA 140-220)

Catalog No. ABIN7216874

The Rabbit Polyclonal anti-RPS3 antibody is suitable to detect RPS3 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for RPS3 antibody (AA 140-220) (ABIN7216874)

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-RPS3 Antibody

Binding Specificity: AA 140-220

Purpose: Rabbit Anti-Ribosomal Protein S3 Polyclonal Antibody

Specificity: Ribosomal Protein S3 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Ribosomal Protein S3 at AA range: 140-220

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RPS3

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Alternative Name: Ribosomal Protein S3

Background: RPS3, OK/SW-cl.26, 40S ribosomal protein S3Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS3 encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. 40S ribosomal protein S3 belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 30kD

Gene ID: 6188

UniProt: P23396

Pathways: Positive Regulation of Endopeptidase Activity