RNF111 antibody (AA 870-950)

Catalog No. ABIN7223318

The Rabbit Polyclonal anti-RNF111 antibody is suitable to detect RNF111 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for RNF111 antibody (AA 870-950) (ABIN7223318)

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF111 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF111 Antibody

Binding Specificity: AA 870-950

Purpose: Rabbit Anti-Arkadia Polyclonal Antibody

Specificity: Arkadia Polyclonal Antibody detects endogenous levels of Arkadia protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Arkadia at AA range: 870-950

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF111

Target: RNF111 (Ring Finger Protein 111 (RNF111))

Alternative Name: Arkadia

Background: RNF111, E3 ubiquitin-protein ligase Arkadia, RING finger protein 111The protein encoded by RNF111 (ring finger protein 111) is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF)-beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, RNF111 plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of RNF111 results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 110kD

Gene ID: 54778

UniProt: Q6ZNA4