RNF14 antibody (AA 330-410)

Catalog No. ABIN7226042

The Rabbit Polyclonal anti-RNF14 antibody is suitable to detect RNF14 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for RNF14 antibody (AA 330-410) (ABIN7226042)

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF14 Antibody

Binding Specificity: AA 330-410

Purpose: ARA54 Polyclonal Antibody

Specificity: ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human ARA54 at AA range: 330-410

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RNF14

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Alternative Name: ARA54

Background: Rabbit Anti-ARA54 Polyclonal Antibody,RNF14, ARA54, HRIHFB2038, E3 ubiquitin-protein ligase RNF14, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein,The protein encoded by RNF14 (ring finger protein 14) contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of RNF14 has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported.,E3 ubiquitin-protein ligase RNF14

Gene ID: 9604

UniProt: Q9UBS8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling