RNF14 antibody (AA 330-410)

Catalog No. ABIN7226042

The Rabbit Polyclonal anti-RNF14 antibody is suitable to detect RNF14 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for RNF14 antibody (AA 330-410) (ABIN7226042)

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF14 Antibody

Binding Specificity: AA 330-410

Purpose: Rabbit Anti-ARA54 Polyclonal Antibody

Specificity: ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human ARA54 at AA range: 330-410

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF14

Target: RNF14 (Ring Finger Protein 14 (RNF14))

Alternative Name: ARA54

Background: RNF14, ARA54, HRIHFB2038, E3 ubiquitin-protein ligase RNF14, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 proteinThe protein encoded by RNF14 (ring finger protein 14) contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of RNF14 has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Molecular Weight: 50kD

Gene ID: 9604

UniProt: Q9UBS8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling