RNF149 antibody (AA 180-260)

Catalog No. ABIN7221232

The Rabbit Polyclonal anti-RNF149 antibody is suitable to detect RNF149 in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for RNF149 antibody (AA 180-260) (ABIN7221232)

Target: RNF149 (Ring Finger Protein 149 (RNF149))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF149 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RNF149 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-RNF149 Polyclonal Antibody

Specificity: RNF149 Polyclonal Antibody detects endogenous levels of RNF149 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RNF149 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF149

Target: RNF149 (Ring Finger Protein 149 (RNF149))

Alternative Name: RNF149

Background: RNF149, DNAPTP2, E3 ubiquitin-protein ligase RNF149, DNA polymerase-transactivated protein 2, RING finger protein 149RNF149 (Ring Finger Protein 149) is a Protein Coding gene. GO annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF167. 3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.

Gene ID: 284996

UniProt: Q8NC42