RNF185 antibody (AA 121-170)

Catalog No. ABIN7231102

The Rabbit Polyclonal anti-RNF185 antibody (ABIN7231102) specifically detects RNF185 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RNF185 antibody (AA 121-170) (ABIN7231102)

Target: RNF185 (Ring Finger Protein 185 (RNF185))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF185 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RNF185 Antibody

Binding Specificity: AA 121-170

Purpose: Rabbit Anti-RN185 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RN185 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RN185 protein at AA range: 121-170

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF185

Target: RNF185 (Ring Finger Protein 185 (RNF185))

Alternative Name: RN185

Background: E3 ubiquitin-protein ligase RNF185, RING finger protein 185, RING-type E3 ubiquitin transferase RNF185RNF185 (Ring Finger Protein 185) is a Protein Coding gene. Diseases associated with RNF185 include Cystic Fibrosis. Among its related pathways are Protein processing in endoplasmic reticulum and HIV Life Cycle. GO annotations related to this gene include ligase activity. An important paralog of this gene is INPP5J. E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.

Molecular Weight: 21kD

Gene ID: 91445

UniProt: Q96GF1