RSK1/2/3/4 (AA 191-240), (pSer221) antibody

Catalog No. ABIN1532079

Product Details

Target: RSK1/2/3/4

Binding Specificity: AA 191-240, pSer221

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Specificity:

RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.

PhosphorylationH:S221/227/S218/232 M:S221/227/S218/252

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:40000

Comment:

Unigene-Number: Hs.149957, Hs.445387, Hs.655277, Hs.368153 (NCBI Gene Symbol: RPS6KA1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details

Target: RSK1/2/3/4

Background:

Synonyms: S6K-alpha 1, 90 kDa ribosomal protein S6 kinase 1, p90-RSK 1, Ribosomal S6 kinase 1, RSK-1, pp90RSK1, p90S6K, MAP kinase-activated protein kinase 1a, MAPKAPK1A/ S6K-alpha 3, 90 kDa ribosomal protein S6 kinase 3, p90-RSK 3, Ribosomal S6 kinase 2, RSK-2, pp

NCBI Gene Symbol: RPS6KA1

Molecular Weight: 82 kDa

Gene ID: 6195, 6197, 6196

OMIM: 601684

UniProt: Q15418, P51812, Q15349, Q9UK32