RUFY1 antibody (AA 110-190)

Catalog No. ABIN7225138

The Rabbit Polyclonal anti-RUFY1 antibody is suitable to detect RUFY1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for RUFY1 antibody (AA 110-190) (ABIN7225138)

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUFY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUFY1 Antibody

Binding Specificity: AA 110-190

Purpose: Rabbit Anti-RUFY1 Polyclonal Antibody

Specificity: RUFY1 Polyclonal Antibody detects endogenous levels of RUFY1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human RUFY1 at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RUFY1

Target: RUFY1 (RUN and FYVE Domain Containing 1 (RUFY1))

Alternative Name: RUFY1

Background: RUFY1, RABIP4, ZFYVE12, RUN and FYVE domain-containing protein 1, FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, Zinc finger FYVE domain-containing protein 12RUFY1 (RUN and FYVE domain containing 1) encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for RUFY1.

Molecular Weight: 80kD

Gene ID: 80230

UniProt: Q96T51