RUNX1 antibody (pSer435)

Catalog No. ABIN7225570

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RUNX1 antibody (pSer435) (ABIN7225570)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX1 Antibody

Binding Specificity: pSer435

Purpose: RUNX1 (phospho Ser435) Polyclonal Antibody

Specificity: Phospho-RUNX1 (S435) Polyclonal Antibody detects endogenous levels of RUNX1 protein only when phosphorylated at S435.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human RUNX1 Phospho-Ser435

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background: Rabbit Anti-RUNX1 (phospho Ser435) Polyclonal Antibody,RUNX1, AML1, CBFA2, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha,Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by CBF2alpha (runt related transcription factor 1, RUNX1) represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for CBF2alpha.,Runt-related transcription factor 1

Molecular Weight: observerd band 53kDa

Gene ID: 861

UniProt: Q01196