SRF antibody (pSer77)

Catalog No. ABIN7224468

The Rabbit Polyclonal anti-SRF antibody (ABIN7224468) specifically detects SRF in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for SRF antibody (pSer77) (ABIN7224468)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SRF Antibody

Binding Specificity: pSer77

Purpose: Rabbit Anti-SRF (phospho Ser77) Polyclonal Antibody

Specificity: Phospho-SRF (S77) Polyclonal Antibody detects endogenous levels of SRF protein only when phosphorylated at S77.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SRF Phospho-Ser77

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background: SRF, Serum response factor, SRFSRF (serum response factor) encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. SRF is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for SRF.

Molecular Weight: 52kD

Gene ID: 6722

UniProt: P11831