SIAH1/2 antibody (AA 160-240)

Catalog No. ABIN7217010

The Rabbit Polyclonal anti-SIAH1/2 antibody (ABIN7217010) specifically detects SIAH1/2 in WB, IHC, IF and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIAH1/2 antibody (AA 160-240) (ABIN7217010)

Target: SIAH1/2 (Seven in Absentia Homolog 1/2 (SIAH1/2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH1/2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-SIAH1/2 Antibody

Binding Specificity: AA 160-240

Purpose: Rabbit Anti-Siah-1/2 Polyclonal Antibody

Specificity: Siah-1/2 Polyclonal Antibody detects endogenous levels of Siah-1/2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Siah-1/2 at AA range: 160-240

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SIAH1/2

Target: SIAH1/2 (Seven in Absentia Homolog 1/2 (SIAH1/2))

Alternative Name: Siah-1/2

Background: SIAH1, HUMSIAH, E3 ubiquitin-protein ligase SIAH1, Seven in absentia homolog 1, Siah-1, Siah-1a, SIAH2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2, hSiah2SIAH1 encodes E3 ubiquitin-protein ligase SIAH1 that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Molecular Weight: 34kD

Gene ID: 6477, 6478

UniProt: Q8IUQ4, O43255