Shugoshin antibody (pSer14)

Catalog No. ABIN7219856

This Rabbit Polyclonal antibody specifically detects Shugoshin in ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Shugoshin antibody (pSer14) (ABIN7219856)

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Shugoshin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Shugoshin Antibody

Binding Specificity: pSer14

Purpose: Rabbit Anti-Sgo1 (phospho Ser14) Polyclonal Antibody

Specificity: Phospho-Sgo1 (S14) Polyclonal Antibody detects endogenous levels of Sgo1 protein only when phosphorylated at S14.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Sgo1 Phospho-Ser14

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Shugoshin

Target: Shugoshin (SGOL1) (Shugoshin 1 (SGOL1))

Alternative Name: Sgo1

Background: SGOL1, SGO1, Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85Shugoshin-like 1 encoded by SGO1 is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID: 18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID: 25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

Gene ID: 151648

UniProt: Q5FBB7

Pathways: Maintenance of Protein Location