SIAH2 antibody (AA 210-290)

Catalog No. ABIN7217012

The Rabbit Polyclonal anti-SIAH2 antibody (ABIN7217012) specifically detects SIAH2 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIAH2 antibody (AA 210-290) (ABIN7217012)

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIAH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SIAH2 Antibody

Binding Specificity: AA 210-290

Purpose: Rabbit Anti-Siah-2 Polyclonal Antibody

Specificity: Siah-2 Polyclonal Antibody detects endogenous levels of Siah-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Siah-2 at AA range: 210-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SIAH2

Target: SIAH2 (Siah E3 Ubiquitin Protein Ligase 2 (SIAH2))

Alternative Name: Siah-2

Background: SIAH2, E3 ubiquitin-protein ligase SIAH2, Seven in absentia homolog 2, Siah-2, hSiah2SIAH2 encodes E3 ubiquitin-protein ligase SIAH2 that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.

Molecular Weight: 38kD

Gene ID: 6478

UniProt: O43255