SIRT1 antibody (pSer47)

Catalog No. ABIN7220712

This Rabbit Polyclonal antibody specifically detects SIRT1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for SIRT1 antibody (pSer47) (ABIN7220712)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SIRT1 Antibody

Binding Specificity: pSer47

Purpose: SIRT1 (phospho Ser47) Polyclonal Antibody

Specificity: Phospho-SIRT1 (S47) Polyclonal Antibody detects endogenous levels of SIRT1 protein only when phosphorylated at S47.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SIRT1 Phospho-Ser47

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: Rabbit Anti-SIRT1 (phospho Ser47) Polyclonal Antibody,SIRT1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIR2,SIRT1 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The sirtuin 1 encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.,NAD-dependent protein deacetylase sirtuin-1

Molecular Weight: observerd band 85-110kDa

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling