SSB antibody (pSer366)

Catalog No. ABIN7224496

The Rabbit Polyclonal anti-SSB antibody is suitable to detect SSB in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for SSB antibody (pSer366) (ABIN7224496)

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SSB Antibody

Binding Specificity: pSer366

Purpose: Rabbit Anti-SSB (phospho Ser366) Polyclonal Antibody

Specificity: Phospho-SSB (S366) Polyclonal Antibody detects endogenous levels of SSB protein only when phosphorylated at S366.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SSB Phospho-Ser366

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SSB

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Alternative Name: SSB

Background: SSB, Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-BThe protein encoded by SSB (Sjogren syndrome antigen B) is involved in diverse aspects of RNA metabolism, including binding and protecting poly (U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.

Molecular Weight: 47kD

Gene ID: 6741

UniProt: P05455