ATP1A1 antibody (pSer16)

Catalog No. ABIN7218818

This Rabbit Polyclonal antibody specifically detects ATP1A1 in WB, ELISA and IF. It exhibits reactivity toward Rat.

Quick Overview for ATP1A1 antibody (pSer16) (ABIN7218818)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-ATP1A1 Antibody

Binding Specificity: pSer16

Purpose: Na+/K+-ATPase α1 (phospho Ser23) Polyclonal Antibody

Specificity: Phospho-Na+/K+-ATPase α1 (S16) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at S16.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Na+/K+-ATPase alpha1 Phospho-Ser16

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: Na+/K+-ATPase alpha1

Background: Rabbit Anti-Na+/K+-ATPase α1 (phospho Ser23) Polyclonal Antibody,ATP1A1, Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1,The ATPase Na+/K+ transporting subunit alpha 1 encoded by ATP1A1 belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.,Sodium potassium-transporting ATPase subunit alpha-1

Molecular Weight: observerd band 113kDa

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process