SPHK2 antibody

Catalog No. ABIN7223680

The Rabbit Polyclonal anti-SPHK2 antibody is suitable to detect SPHK2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for SPHK2 antibody (ABIN7223680)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SPHK2 Antibody

Purpose: Rabbit Anti-SphK2 Polyclonal Antibody

Specificity: SphK2 Polyclonal Antibody detects endogenous levels of SphK2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human SphK2 around the non-phosphorylation site of T614

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Alternative Name: SphK2

Background: SPHK2, Sphingosine kinase 2, SK 2, SPK 2SPHK2 (sphingosine kinase 2) encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for SPHK2.

Molecular Weight: 70kD

Gene ID: 56848

UniProt: Q9NRA0

Pathways: VEGF Signaling