SHB antibody (pTyr246)

Catalog No. ABIN7213118

The Rabbit Polyclonal anti-SHB antibody (ABIN7213118) specifically detects SHB in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for SHB antibody (pTyr246) (ABIN7213118)

Target: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SHB Antibody

Binding Specificity: pTyr246

Purpose: Rabbit Anti-Shb (phospho Tyr246) Polyclonal Antibody

Specificity: Phospho-Shb (Y246) Polyclonal Antibody detects endogenous levels of Shb protein only when phosphorylated at Y246.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Shb Phospho-Tyr246

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SHB

Target: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Alternative Name: Shb

Background: SHB, SH2 domain-containing adapter protein BSHB (SH2 homology protein in B/ beta -cells) is a 55-59 kDa cytoplasmic adaptor protein that serves as a link between phosphotyrosine residues and downstream signaling pathways. SHB is ubiquitously expressed, and binds tyrosine kinase receptors such as FGFR1, VEGFR2, and the TCR (zeta -chain) following their activation. It is highly modular, and through a variety of motifs, is able to bind multiple, structurally unrelated proteins that collectively generate a signal transduction network. Human SHB is 509 amino acids (aa) in length. It contains an N-terminal Pro-rich region that binds SH3 domain-containing proteins (aa 19-45), a central PTB domain that binds select aa motifs, and a C-terminal SH2 domain that interacts with phosphotyrosines (aa 410-504). There are at least nine utilized phosphorylation sites. There are two isoform variants. One is 66-68 kDa in size, and possesses an 87 aa Pro-rich N-terminal extension. This MW may increase to 77-80 kDa following posttranslational processing. A second variant shows an 18 aa substitution for aa 280-509. Over aa 2-128, human SHB shares 91 % aa identity with mouse SHB.

Molecular Weight: 55kD

Gene ID: 6461

UniProt: Q15464