SOAT1 antibody (AA 190-270)

Catalog No. ABIN7213440

The Rabbit Polyclonal anti-SOAT1 antibody is suitable to detect SOAT1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for SOAT1 antibody (AA 190-270) (ABIN7213440)

Target: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SOAT1 Antibody

Binding Specificity: AA 190-270

Purpose: Rabbit Anti-ACAT-1 Polyclonal Antibody

Specificity: ACAT-1 Polyclonal Antibody detects endogenous levels of ACAT-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human ACAT-1 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SOAT1

Target: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Alternative Name: ACAT-1

Background: ACAT1, ACAT, MAT, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2ACAT1 encodes a mitochondrially localized enzyme (acetyl-CoA acetyltransferase 1) that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in ACAT1 are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Molecular Weight: 45kD

Gene ID: 38

UniProt: P24752