SREBF1 antibody (pSer439)

Catalog No. ABIN7224464

This anti-SREBF1 antibody is a Rabbit Polyclonal antibody detecting SREBF1 in WB, ELISA and IHC (p). Suitable for Human and Rat.

Quick Overview for SREBF1 antibody (pSer439) (ABIN7224464)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SREBF1 Antibody

Binding Specificity: pSer439

Purpose: SREBP-1 (phospho Ser439) Polyclonal Antibody

Specificity: Phospho-SREBP-1 (S439) Polyclonal Antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at S439.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human SREBP-1 Phospho-Ser439

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBP-1

Background: Rabbit Anti-SREBP-1 (phospho Ser439) Polyclonal Antibody,SREBF1, BHLHD1, SREBP1, Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1,SREBF1 (sterol regulatory element binding transcription factor 1) encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. SREBF1 is located within the Smith-Magenis syndrome region on chromosome 17.,Sterol regulatory element-binding protein 1

Molecular Weight: observerd band 122kDa

Gene ID: 6720

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha