STON1 antibody (AA 660-740)

Catalog No. ABIN7219210

The Rabbit Polyclonal anti-STON1 antibody is suitable to detect STON1 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for STON1 antibody (AA 660-740) (ABIN7219210)

Target: STON1 (Stonin 1 (STON1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STON1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-STON1 Antibody

Binding Specificity: AA 660-740

Purpose: Rabbit Anti-Stonin-1 Polyclonal Antibody

Specificity: Stonin-1 Polyclonal Antibody detects endogenous levels of Stonin-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Stonin-1 at AA range: 660-740

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for STON1

Target: STON1 (Stonin 1 (STON1))

Alternative Name: Stonin-1

Background: STON1, SALF, SBLF, STN1, Stonin-1, Stoned B-like factorEndocytosis of cell surface proteins is mediated by a complex molecular machinery that assembles on the inner surface of the plasma membrane. STON1 encodes one of two human homologs of the Drosophila melanogaster stoned B protein. Stonin-1 is related to components of the endocytic machinery and exhibits a modular structure consisting of an N-terminal proline-rich domain, a central region of homology specific to the human stoned B-like proteins, and a C-terminal region homologous to the mu subunits of adaptor protein (AP) complexes. Read-through transcription of this gene into the neighboring downstream gene, which encodes TFIIA-alpha/beta-like factor, generates a transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Alternative splicing results in multiple transcript variants.

Molecular Weight: 83kD

Gene ID: 11037

UniProt: Q9Y6Q2