SAE1 antibody (AA 190-270)

Catalog No. ABIN7216936

The Rabbit Polyclonal anti-SAE1 antibody (ABIN7216936) specifically detects SAE1 in WB, ELISA, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SAE1 antibody (AA 190-270) (ABIN7216936)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-SAE1 Antibody

Binding Specificity: AA 190-270

Purpose: Rabbit Anti-SAE1 Polyclonal Antibody

Specificity: SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human SAE1 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background: SAE1, AOS1, SUA1, UBLE1A, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1APosttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.

Molecular Weight: 38kD

Gene ID: 10055

UniProt: Q9UBE0