Supervillin antibody

Catalog No. ABIN7231602

This Rabbit Polyclonal antibody specifically detects Supervillin in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for Supervillin antibody (ABIN7231602)

Target: Supervillin (SVIL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Supervillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Supervillin Antibody

Purpose: Rabbit Anti-SVIL Polyclonal Antibody

Specificity: The antibody detects endogenous levels of SVIL protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human SVIL protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Supervillin

Target: Supervillin (SVIL)

Alternative Name: SVIL

Background: SupervillinSVIL (Supervillin) encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. SVIL product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Several transcript variants encoding different isoforms of supervillin have been described.

Molecular Weight: 243kD

Gene ID: 6840

UniProt: O95425