STX1A antibody (pSer14)

Catalog No. ABIN7224548

This Rabbit Polyclonal antibody specifically detects STX1A in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for STX1A antibody (pSer14) (ABIN7224548)

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-STX1A Antibody

Binding Specificity: pSer14

Purpose: Rabbit Anti-Syntaxin 1 (phospho Ser14) Polyclonal Antibody

Specificity: Phospho-Syntaxin 1 (S14) Polyclonal Antibody detects endogenous levels of Syntaxin 1 protein only when phosphorylated at S14.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Syntaxin 1 Phospho-Ser14

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for STX1A

Target: STX1A (Syntaxin 1A (Brain) (STX1A))

Alternative Name: Syntaxin 1

Background: STX1A, STX1, Syntaxin-1A, Neuron-specific antigen HPC-1STX1A (syntaxin 1A) encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for STX1A.

Molecular Weight: 35kD

Gene ID: 6804

UniProt: Q16623

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport