T-Bet antibody

Catalog No. ABIN7231732

The Rabbit Polyclonal anti-T-Bet antibody is suitable to detect T-Bet in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for T-Bet antibody (ABIN7231732)

Target: T-Bet

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-T-Bet Antibody

Purpose: Rabbit Anti-TBX21 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of TBX21 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human TBX21 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background: T-box transcription factor TBX21, T-box protein 21, T-cell-specific T-box transcription factor T-bet, Transcription factor TBLYMTBX21 gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Molecular Weight: 58kD

Gene ID: 30009

UniProt: Q9UL17