TGIF1 antibody (C-Term)

Catalog No. ABIN7224686

This anti-TGIF1 antibody is a Rabbit Polyclonal antibody detecting TGIF1 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for TGIF1 antibody (C-Term) (ABIN7224686)

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGIF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TGIF1 Antibody

Binding Specificity: C-Term

Purpose: TGIF Polyclonal Antibody

Specificity: TGIF Polyclonal Antibody detects endogenous levels of TGIF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human TGIF

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TGIF1

Target: TGIF1 (TGFB-Induced Factor Homeobox 1 (TGIF1))

Alternative Name: TGIF

Background: Rabbit Anti-TGIF Polyclonal Antibody,TGIF1, TGIF, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1,The protein encoded by TGIF1 (TGFB induced factor homeobox 1) is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in TGIF1 are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.,Homeobox protein TGIF1

Molecular Weight: observerd band 43kDa

Gene ID: 7050

UniProt: Q15583

Pathways: Retinoic Acid Receptor Signaling Pathway, Chromatin Binding, Tube Formation