TOP3B antibody (AA 30-110)

Catalog No. ABIN7217354

The Rabbit Polyclonal anti-TOP3B antibody is suitable to detect TOP3B in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for TOP3B antibody (AA 30-110) (ABIN7217354)

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TOP3B Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-Topo IIIβ-1 Polyclonal Antibody

Specificity: Topo IIIβ-1 Polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Topo IIIbeta-1 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TOP3B

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Alternative Name: Topo IIIbeta-1

Background: TOP3B, TOP3B1, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1TOP3B encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Molecular Weight: 96kD

Gene ID: 8940

UniProt: O95985