TFE3 antibody (AA 70-150)

Catalog No. ABIN7224672

This Rabbit Polyclonal antibody specifically detects TFE3 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TFE3 antibody (AA 70-150) (ABIN7224672)

Target: TFE3 (Transcription Factor E3 (TFE3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFE3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TFE3 Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-TFE3 Polyclonal Antibody

Specificity: TFE3 Polyclonal Antibody detects endogenous levels of TFE3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TFE3 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TFE3

Target: TFE3 (Transcription Factor E3 (TFE3))

Alternative Name: TFE3

Background: TFE3, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33TFE3 (transcription factor binding to IGHM enhancer 3) encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. TFE3 may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants.

Molecular Weight: 62kD

Gene ID: 7030

UniProt: P19532