Mafg antibody

Catalog No. ABIN7229506

The Rabbit Polyclonal anti-Mafg antibody is suitable to detect Mafg in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Mafg antibody (ABIN7229506)

Target: Mafg (Transcription Factor MafG (Mafg))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mafg antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Mafg Antibody

Purpose: Rabbit Anti-MAFG Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MAFG protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MAFG protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Mafg

Target: Mafg (Transcription Factor MafG (Mafg))

Alternative Name: MAFG

Background: V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAFGlobin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al. 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877, MafG, or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).

Molecular Weight: 17kD

Gene ID: 4097

UniProt: O15525