MAFK antibody

Catalog No. ABIN7229508

The Rabbit Polyclonal anti-MAFK antibody (ABIN7229508) specifically detects MAFK in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for MAFK antibody (ABIN7229508)

Target: MAFK (Transcription Factor MafK (MAFK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAFK Antibody

Purpose: Rabbit Anti-MAFK Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MAFK protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MAFK protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAFK

Target: MAFK (Transcription Factor MafK (MAFK))

Alternative Name: MAFK

Background: Transcription factor MafK, Erythroid transcription factor NF-E2 p18 subunitThe developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD, MIM 609806) and ferrochelatase (FECH, MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites. MAFK (MAF BZIP Transcription Factor K) is a Protein Coding gene. Diseases associated with MAFK include Fibrosarcoma. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Factors involved in megakaryocyte development and platelet production.

Molecular Weight: 17kD

Gene ID: 7975

UniProt: O60675