TLE2 antibody (AA 180-260)

Catalog No. ABIN7224714

The Rabbit Polyclonal anti-TLE2 antibody is suitable to detect TLE2 in samples from Human. It has been validated for WB, ELISA and IHC (p).

Quick Overview for TLE2 antibody (AA 180-260) (ABIN7224714)

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TLE2 Antibody

Binding Specificity: AA 180-260

Purpose: TLE2 Polyclonal Antibody

Specificity: TLE2 Polyclonal Antibody detects endogenous levels of TLE2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human TLE2 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for TLE2

Target: TLE2 (Transducin-Like Enhancer Protein 2 (TLE2))

Alternative Name: TLE2

Background: Rabbit Anti-TLE2 Polyclonal Antibody,TLE2, Transducin-like enhancer protein 2, Enhancer of split groucho-like protein 2, ESG2,Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho. TLEs contain several WD-repeats implicated in protein-protein interaction. TLEs are transcriptional co-repressors that bind to many transcription factors such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3. TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions.,Transducin-like enhancer protein 2

Molecular Weight: observerd band 110kDa

Gene ID: 7089

UniProt: Q04725

Pathways: WNT Signaling