TLE4 antibody (AA 130-210)

Catalog No. ABIN7217332

The Rabbit Polyclonal anti-TLE4 antibody (ABIN7217332) specifically detects TLE4 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for TLE4 antibody (AA 130-210) (ABIN7217332)

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TLE4 Antibody

Binding Specificity: AA 130-210

Purpose: Rabbit Anti-TLE4 Polyclonal Antibody

Specificity: TLE4 Polyclonal Antibody detects endogenous levels of TLE4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TLE4 at AA range: 130-210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TLE4

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Alternative Name: TLE4

Background: TLE4, KIAA1261, Transducin-like enhancer protein 4TLE4 (Transducin Like Enhancer Of Split 4) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by NOTCH1. Gene Ontology (GO) annotations related to this gene include chromatin binding and transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding. An important paralog of this gene is AES. Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).

Molecular Weight: 83kD

Gene ID: 7091

UniProt: Q04727

Pathways: WNT Signaling