TFF1 antibody

Catalog No. ABIN7212348

The Mouse Monoclonal anti-TFF1 antibody (Clone 5E3) (ABIN7212348) specifically detects TFF1 in IHC, WB and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TFF1 antibody (ABIN7212348)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Clone: 5E3

Product Details anti-TFF1 Antibody

Purpose: Mouse Anti-HP-1α Mouse Monoclonal Antibody (5E3)

Specificity: The antibody detects endogenous HP-1α protein.

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant Protein of HP-1alpha

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TFF1

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: HP-1alpha

Background: Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alphaCBX5 (chromobox 5) encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. CBX5 has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 22kD

Gene ID: 23468

UniProt: P45973

Pathways: EGFR Signaling Pathway