TRIM3 antibody (AA 1-80)

Catalog No. ABIN7219082

The Rabbit Polyclonal anti-TRIM3 antibody (ABIN7219082) specifically detects TRIM3 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TRIM3 antibody (AA 1-80) (ABIN7219082)

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TRIM3 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-TRIM3 Polyclonal Antibody

Specificity: TRIM3 Polyclonal Antibody detects endogenous levels of TRIM3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human TRIM3 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TRIM3

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Alternative Name: TRIM3

Background: TRIM3, BERP, RNF22, RNF97, Tripartite motif-containing protein 3, Brain-expressed RING finger protein, RING finger protein 22, RING finger protein 97The tripartite motif containing 3 encoded by TRIM3 is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.

Molecular Weight: 81kD

Gene ID: 10612

UniProt: O75382