Tropomyosin antibody (N-Term)

Catalog No. ABIN7217388

This Rabbit Polyclonal antibody specifically detects Tropomyosin in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Tropomyosin antibody (N-Term) (ABIN7217388)

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tropomyosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Tropomyosin Antibody

Binding Specificity: N-Term

Purpose: Rabbit Anti-Tropomyosin α Polyclonal Antibody

Specificity: Tropomyosin α Polyclonal Antibody detects endogenous levels of Tropomyosin α protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Tropomyosin alpha

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Tropomyosin

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Alternative Name: Tropomyosin alpha

Background: TPM1, C15orf13, TMSA, Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The tropomyosin alpha-1 chain is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.

Molecular Weight: 35kD

Gene ID: 7168

UniProt: P09493

Pathways: Regulation of Actin Filament Polymerization