TNNI2 antibody

Catalog No. ABIN7224730

The Rabbit Polyclonal anti-TNNI2 antibody (ABIN7224730) specifically detects TNNI2 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TNNI2 antibody (ABIN7224730)

Target: TNNI2 (Fast Skeletal Troponin I (TNNI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-TNNI2 Antibody

Purpose: Rabbit Anti-Troponin I-C Polyclonal Antibody

Specificity: Troponin I-C Polyclonal Antibody detects endogenous levels of Troponin I-C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Troponin I-C around the non-phosphorylation site of T142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TNNI2

Target: TNNI2 (Fast Skeletal Troponin I (TNNI2))

Alternative Name: Troponin I-C

Background: TNNI3, TNNC1, Troponin I, cardiac muscle, Cardiac troponin ITroponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. NNI3 (troponin I3, cardiac type) encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in TNNI3 cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Molecular Weight: 28kD

Gene ID: 7137

UniProt: P19429